The Ryanodine Receptors Ca 2 + Release Channels: Cellular Redox Sensors?
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چکیده
منابع مشابه
Ruthenium red modifies the cardiac and skeletal muscle Ca(2+) release channels (ryanodine receptors) by multiple mechanisms.
The effects of ruthenium red (RR) on the skeletal and cardiac muscle ryanodine receptors (RyRs) were studied in vesicle-Ca(2+) flux, [(3)H]ryanodine binding, and single channel measurements. In vesicle-Ca(2+) flux measurements, RR was more effective in inhibiting RyRs at 0.2 microM than 20 microM free Ca(2+). [(3)H]Ryanodine binding measurements suggested noncompetitive interactions between RR ...
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The type 1 ryanodine receptor (RyR1) from rabbit skeletal muscle displayed two distinct degrees of response to cytoplasmic Ca(2+) [high- and low-open probability (P(o)) channels]. Here, we examined the effects of adenine nucleotides and caffeine on these channels and their modulations by sulfhydryl reagents. High-P(o) channels showed biphasic Ca(2+) dependence and were activated by adenine nucl...
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Single-channel and [ 3 H]ryanodine binding experiments were carried out to examine the effects of imperatoxin activator (IpTx a ), a 33 amino acid peptide isolated from the venom of the African scorpion Pandinus imperator , on rabbit skeletal and canine cardiac muscle Ca 2 1 release channels (CRCs). Single channel currents from purified CRCs incorporated into planar lipid bilayers were recorded...
متن کاملCoupled gating between cardiac calcium release channels (ryanodine receptors).
Excitation-contraction coupling in heart muscle requires the activation of Ca(2+)-release channels/type 2 ryanodine receptors (RyR2s) by Ca(2+) influx. RyR2s are arranged on the sarcoplasmic reticular membrane in closely packed arrays such that their large cytoplasmic domains contact one another. We now show that multiple RyR2s can be isolated under conditions such that they remain physically c...
متن کاملCalcineurin regulates ryanodine receptor/Ca(2+)-release channels in rat heart.
The present study was undertaken to examine the physical and physiological interaction of protein phosphatase 2B, calcineurin, with the ryanodine receptor (RyR) in rat cardiac tissue and neonatal cardiomyocytes. The presence of calcineurin, the RyR and FK506-binding protein (FKBP)12.6 in rat cardiac sarcoplasmic reticulum (SR) was identified by Western blot analysis. The possible interactions b...
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ژورنال
عنوان ژورنال: IUBMB Life (International Union of Biochemistry and Molecular Biology: Life)
سال: 2005
ISSN: 1521-6543,1521-6551
DOI: 10.1080/15216540500092328